Bioscience Protocol List 

A

Agarose DNA Gels

Alpha-1-Antitrypsin purification from plasma

APMA Activation of MMPs 

B

Bradford Assay

C

Cross Linking - Glutaraldehyde

D

Decontamination of Ethidium Bromide Waste (Activated Charcoal Method)

Decontamination of Ethidium Bromide Waste (Tea Bag Method)

Densitometry of Gels

Dialysis to Buffer Exchange Proteins

6 x DNA Loading Buffer (Orange G/Ficoll based)

E

Ethanol Precipitation of DNA

EXOSAP cleanup of PCR products (For sequencing)

F

G

Glycerol Stock Preparation 

H

I

Induced Sputum (Obtaining + Processing)

J

K

L

Loading Buffer - Native Page

M

MTT Proliferation/Viability Assay

N

Native Page (Tris Glycine)

Neutrophil Isolation

O

P

PBS Buffer (10 x) 

PCR - Touchdown PCR

Plasma preparation from whole blood

Pottasium Phosphate Buffer

Q

R

S

3 x SDS Page Sample Loading Buffer (Lammeli loading stock) 

Silver Staining

Skin Prick Test

Sodium Phosphate Buffer

T

TAE (x50) Buffer 

Transformation of Bacteria 

Transwell Migration Assay

W

X

Y

Z

Zymography - Casein 

Zymography - Gelatin