activation of Matrix Metalloproteinases
fresh 10mM stock of APMA (
4-aminophenylmercuric acetate) using
(This must be done prior to activation
stock APMA 4x in TCNB buffer – 50mM Tris, 10mM CaCl2, 150mM
NaCl, 0.05% Brij35, pH7.5
pH to 7.5 using HCl
sample 1 in 5 with APMA solution
at 37C dependant on MMP.
requires 2 hours whilst MMP-9 requires 5 hours
Please e-mail us times for other MMPs.