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Step by step methodologies

 
 

Sodium Phosphate Buffer

Method to make up a 0.1M Sodium Phospate Buffer at a required pH

1. Make up 1M Na2HPO4 stock

2. Make up 1M NaH2PO4 stock

3. Use the following table to mix the stock buffers together to attain the required pH at 25C

pH

1M Na2HPO4

 

1M NaH2PO4

 

5.8

7.9

92.1

6.0

12.0

88.0

6.2

17.8

82.2

6.4

25.5

74.5

6.6

35.2

64.8

6.8

46.3

53.7

7.0

57.7

42.3

7.2

68.4

31.6

7.4

77.4

22.6

7.6

84.5

15.5

7.8

89.6

10.4

8.0

93.2

6.8

4. Add 900mls distilled H20