Sodium Phosphate
Buffer
Method to make up a 0.1M
Sodium Phospate Buffer at a required pH
1. Make up 1M Na2HPO4 stock
2. Make up 1M NaH2PO4 stock
3. Use the following table to mix the stock
buffers together to attain the required pH at 25C
|
pH
|
1M
Na2HPO4
|
1M
NaH2PO4
|
|
5.8
|
7.9
|
92.1
|
|
6.0
|
12.0
|
88.0
|
|
6.2
|
17.8
|
82.2
|
|
6.4
|
25.5
|
74.5
|
|
6.6
|
35.2
|
64.8
|
|
6.8
|
46.3
|
53.7
|
|
7.0
|
57.7
|
42.3
|
|
7.2
|
68.4
|
31.6
|
|
7.4
|
77.4
|
22.6
|
|
7.6
|
84.5
|
15.5
|
|
7.8
|
89.6
|
10.4
|
|
8.0
|
93.2
|
6.8
|
4. Add 900mls distilled
H20
|